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To do:
Continuously:
Goal of having specific hours in the lab and break experiments down into more routine, manageable chunks. Jin is hereby banned from any more "heroic efforts".

Tomorrow:
24 individual fly extractions
Lunch
24 gDNA validations
Dinner
24 individuals run on lightcycler against homozygous DNA standards.

Order primers from invitrogen.

This week:
test RT-PCR primers:

Cook laying plates
cook 3x agar normal food plates

Generate Celera 3rd instar, do not chemically challenge, freeze in LN2.

extract.
RT-PCR and check pipetting accuracy.

(lisa and ronald offered to help)

This month:
Plan preliminary willoughby-style 3rd Instar exposures. (day by day plan)

Make a graph of the backcross survivors which shows the raw data. No averages. Just number into challenge vial and number of surviving adults per vial. (cf emily remnant)

Remind P to email T.

Next month:
Reciprocal cross to finally nail down if F1 resistance is dominant or recessive for each toxin.

Read up on alpha a resistance, check original papers. Dominant or recessive? monogeneic sure, but what other properties.

"Small consistent efforts. That's what we need. That builds routine. We can build off a low base. Once you get 5 days in a row of good, productive, sensible work, even if it's two hours a day, we can easily extend to 6 days or more. I'd rather have you in the lab for 2 hours a day for 5 days, than seeing you in for 16 hours in one day and then despondent and exhausted for the rest of the week."